Plasmid

Part:BBa_K260000

Designed by: Kaj Bernhardt   Group: iGEM09_BIOTEC_Dresden   (2009-10-17)

pCC2FosM

This is a fosmid based on pCC2Fos from [http://www.epibio.com/item.asp?ID=385 EPICENTRE Biotechnologies]. It contains 38 kb from the human MECP2 genomic locus, with a spectinomycin resistance (SpecR) cassette in the first intron of MECP2.

PCC2Fos-MeCP2-SpecR.jpg

This fosmid is used to accept by recombineering the HincII fragment from BBa_K260016 (inside backbone BBa_K260010). This reaction also transfers the blasticidin resistance gene (BsdR) to generate the new backbone BBa_K260001 (pCC2FosMB), then containing the same BBa_K260016 part.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 332
    Illegal EcoRI site found at 12286
    Illegal EcoRI site found at 14389
    Illegal EcoRI site found at 17525
    Illegal EcoRI site found at 18022
    Illegal EcoRI site found at 19261
    Illegal EcoRI site found at 19847
    Illegal XbaI site found at 2062
    Illegal XbaI site found at 10251
    Illegal XbaI site found at 13089
    Illegal XbaI site found at 24698
    Illegal XbaI site found at 27576
    Illegal XbaI site found at 32165
    Illegal XbaI site found at 32453
    Illegal SpeI site found at 20096
    Illegal SpeI site found at 46280
    Illegal PstI site found at 811
    Illegal PstI site found at 1299
    Illegal PstI site found at 1945
    Illegal PstI site found at 4280
    Illegal PstI site found at 8707
    Illegal PstI site found at 10659
    Illegal PstI site found at 12598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 332
    Illegal EcoRI site found at 12286
    Illegal EcoRI site found at 14389
    Illegal EcoRI site found at 17525
    Illegal EcoRI site found at 18022
    Illegal EcoRI site found at 19261
    Illegal EcoRI site found at 19847
    Illegal NheI site found at 17915
    Illegal NheI site found at 34821
    Illegal SpeI site found at 20096
    Illegal SpeI site found at 46280
    Illegal PstI site found at 811
    Illegal PstI site found at 1299
    Illegal PstI site found at 1945
    Illegal PstI site found at 4280
    Illegal PstI site found at 8707
    Illegal PstI site found at 10659
    Illegal PstI site found at 12598
    Illegal NotI site found at 1
    Illegal NotI site found at 40200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 332
    Illegal EcoRI site found at 12286
    Illegal EcoRI site found at 14389
    Illegal EcoRI site found at 17525
    Illegal EcoRI site found at 18022
    Illegal EcoRI site found at 19261
    Illegal EcoRI site found at 19847
    Illegal BglII site found at 7793
    Illegal BglII site found at 9219
    Illegal BglII site found at 11392
    Illegal BglII site found at 16539
    Illegal BglII site found at 19858
    Illegal BglII site found at 42704
    Illegal BglII site found at 44771
    Illegal BamHI site found at 353
    Illegal BamHI site found at 1253
    Illegal BamHI site found at 1713
    Illegal BamHI site found at 16969
    Illegal BamHI site found at 19393
    Illegal BamHI site found at 21803
    Illegal BamHI site found at 39923
    Illegal XhoI site found at 1638
    Illegal XhoI site found at 5871
    Illegal XhoI site found at 21569
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 332
    Illegal EcoRI site found at 12286
    Illegal EcoRI site found at 14389
    Illegal EcoRI site found at 17525
    Illegal EcoRI site found at 18022
    Illegal EcoRI site found at 19261
    Illegal EcoRI site found at 19847
    Illegal XbaI site found at 2062
    Illegal XbaI site found at 10251
    Illegal XbaI site found at 13089
    Illegal XbaI site found at 24698
    Illegal XbaI site found at 27576
    Illegal XbaI site found at 32165
    Illegal XbaI site found at 32453
    Illegal SpeI site found at 20096
    Illegal SpeI site found at 46280
    Illegal PstI site found at 811
    Illegal PstI site found at 1299
    Illegal PstI site found at 1945
    Illegal PstI site found at 4280
    Illegal PstI site found at 8707
    Illegal PstI site found at 10659
    Illegal PstI site found at 12598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 332
    Illegal EcoRI site found at 12286
    Illegal EcoRI site found at 14389
    Illegal EcoRI site found at 17525
    Illegal EcoRI site found at 18022
    Illegal EcoRI site found at 19261
    Illegal EcoRI site found at 19847
    Illegal XbaI site found at 2062
    Illegal XbaI site found at 10251
    Illegal XbaI site found at 13089
    Illegal XbaI site found at 24698
    Illegal XbaI site found at 27576
    Illegal XbaI site found at 32165
    Illegal XbaI site found at 32453
    Illegal SpeI site found at 20096
    Illegal SpeI site found at 46280
    Illegal PstI site found at 811
    Illegal PstI site found at 1299
    Illegal PstI site found at 1945
    Illegal PstI site found at 4280
    Illegal PstI site found at 8707
    Illegal PstI site found at 10659
    Illegal PstI site found at 12598
    Illegal NgoMIV site found at 11340
    Illegal NgoMIV site found at 11659
    Illegal NgoMIV site found at 30388
    Illegal NgoMIV site found at 31436
    Illegal NgoMIV site found at 42043
    Illegal NgoMIV site found at 42050
    Illegal NgoMIV site found at 42174
    Illegal AgeI site found at 26093
    Illegal AgeI site found at 31515
    Illegal AgeI site found at 43385
    Illegal AgeI site found at 44615
    Illegal AgeI site found at 45508
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1876
    Illegal BsaI site found at 4595
    Illegal BsaI site found at 6331
    Illegal BsaI site found at 13509
    Illegal BsaI site found at 13650
    Illegal BsaI site found at 21125
    Illegal BsaI site found at 21566
    Illegal BsaI.rc site found at 3482
    Illegal BsaI.rc site found at 3741
    Illegal BsaI.rc site found at 8532
    Illegal BsaI.rc site found at 8757
    Illegal BsaI.rc site found at 9068
    Illegal BsaI.rc site found at 9660
    Illegal BsaI.rc site found at 9851
    Illegal SapI site found at 23985
    Illegal SapI site found at 31323
    Illegal SapI site found at 33834
    Illegal SapI site found at 33856
    Illegal SapI site found at 44154
    Illegal SapI site found at 45364
    Illegal SapI.rc site found at 16587
    Illegal SapI.rc site found at 36025
    Illegal SapI.rc site found at 38282


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Categories
Parameters
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